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  • Micro-patterned silicon chip for macromolecular crystallography
    Sample holder for up to 22.500 crystals

For serial crystallography experiments at home sources, synchrotrons and XFELs

  • Periodic arrangement of micropores
  • Pore sizes selectable between 2µm and 50µm
  • Easy loading by "micro-sieve"-approach
  • Most efficient use of sample material
  • Ideally suited for automatic raster scanning
  • Compatible with data collection at room and cryogenic temperatures
  • Mounted on standard magnetic cap, compatible with crystallography beamlines
Chip
Chip

Ultra-low background

  • Fabricated by micro-lithography from single crystalline silicon
  • No background scattering due to single crystalline substrate material
  • Bragg reflections from substrate at well defined orientations only
  • Very low absorption due to 5-10 µm membrane thickness
  • Micro-sieve structure allows efficient removal of excessive mother liquor

Picture on the left side: "X-ray background level originating from the silicon chip" by Roedig, P. et al., licensed under CC BY 4.0. Source: http://www.nature.com/articles/srep10451/figures/3

For serial crystallography experiments at home sources, synchrotrons and XFELs

    A. The mother liquor is pipetted on the chip and fills the microchannels on the front-side of the chip
    B. A small wedge of filter paper touches the lower side of the chip
    C. The filter paper soaks off the liquid through the micro channels
    D. Crystals with sizes larger than the pores are retained and arrange themselves in a periodic way according to the pore structure of the chip
    E. The filter paper is removed from the lower side of the chip
    F. The crystals on the chip are flash-frozen in liquid nitrogen

    During the sample loading process, the chip is placed in a continuous stream of air with a controlled degree of humidity.

    Reference: Roedig, P. et al. A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering. Sci. Rep. 5, 10451; doi: 10.1038/srep10451 (2015). Picture on the right side: "Sample loading procedure" by Roedig, P. et al., licensed under CC BY 4.0 / Desaturated from original
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