A – A small volume of a micro-crystal suspension is pipetted onto the chip membrane
B – A small wedge of filter paper is applied to the bottom side of the chip
C – The filter paper soaks off the liquid through the micro channels
D – Crystals with sizes larger than the pores are retained and arrange themselves in a periodic way according to the pore structure of the chip
E – The filter paper is removed from the lower side of the chip
F – The crystals on the chip can be either flash-frozen in liquid nitrogen or the sample holder can be equipped with a protective cover (not shown)
Important: During the entire sample loading process, it is required to keep the chip in a continuous air-stream providing a controlled relative humidity environment.